Anti-diarrheal and thrombolytic activity of the leaf extract of Phyla nodiflora
DOI:
https://doi.org/10.71193/jpci.20250009Keywords:
Diarrhea, Loperamide, Phyla nodiflora, Streptokinase, Thrombotic disordersAbstract
Phyla nodiflora (PNF), a medicinal plant, may offer a safer, more accessible alternative to synthetic drugs with fewer side effects. This study aimed to investigate the anti-diarrheal and thrombolytic properties of PNF leaf extract using in vivo and in vitro models, respectively. The anti-diarrheal activity of PNF was evaluated in Swiss albino mice using a castor oil-induced diarrhea model. Mice were administered PNF orally at 300 and 600 mg/kg, with loperamide (LOP) (3 mg/kg) as the positive control and distilled water (DW) as the negative control (NC). For thrombolytic activity, an in vitro clot lysis assay was conducted using human blood samples. Our result demonstrated that PNF possesses significant anti-diarrheal effects in a dose-dependent manner, reducing defecation by 27.27% and 63.64% at 300 and 600 mg/kg, respectively (p < 0.05), compared to 72.73% inhibition by LOP. Additionally, PNF exhibited notable clot lysis activity (44.59%), surpassing the NC (8.06%) but lower than streptokinase (STK) (74.25%) (p < 0.005). However, PNF leaf extract shows promising anti-diarrheal and thrombolytic activities, suggesting its potential as a natural therapeutic agent. Further research is needed to clarify its mechanisms and confirm clinical effectiveness.
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Copyright (c) 2025 Nishat Bahadur, Asit Baron Sarker, Dipongkar Ray Sobuj, Feroz Khan Nun, Khadija Akter Khadija Akter, Md. Arif Hossain, Proma Mandal, Farhana Israt Jahan, Tamanna Rashid, Mohammad Aslam, Md. Sakib Al Hasan (Author)
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