Sub-acute Cinnamaldehyde rectal administration induces non-toxic effects in neurological and physiological responses in C57BL/6 mice

Emanuel Bottino; Yesica Paola   Zaio; Natalia Agustina  Saavedra Larralde; Victoria Belén  Occhieppo; Maria Paula Zunino; Andrés Alberto   Ponce

doi:10.71193/jpci.20250015

Authors

Emanuel Bottino Cátedra de Fisiología Humana, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Córdoba, Argentina Author https://orcid.org/0000-0002-0353-7183
Yesica Paola Zaio Cátedra de Química Orgánica y Productos Naturales- Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET), Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba. Córdoba, Argentina Author https://orcid.org/0009-0001-6541-834X
Natalia A. Saavedra Larralde Cátedra de Patología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Córdoba, Argentina Author https://orcid.org/0009-0006-6681-8296
Victoria B. Occhieppo Departamento de Farmacología Otto Orsingher, IFEC-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Córdoba, Argentina Author https://orcid.org/0000-0002-9325-3458
M. Paula Zunino Cátedra de Química Orgánica y Productos Naturales- Instituto Multidisciplinario de Biología Vegetal (IMBIV-CONICET), Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba. Córdoba, Argentina Author https://orcid.org/0000-0002-5320-0036
Andres A. Ponce Cátedra de Fisiología Humana, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. Córdoba, Argentina; Cátedra de Fisiología Humana, Dpto. Académico de Ciencias de la Salud y Educación, Universidad Nacional de la Rioja. La Rioja, Argentina Author https://orcid.org/0000-0002-3322-9456

DOI:

https://doi.org/10.71193/jpci.20250015

Keywords:

Trans-Cinnamaldehyde, intra-rectal, functional observational battery, toxicity, mice

Abstract

Trans-Cinnamaldehyde, the main component of cinnamon essential oil, is widely used in food and traditional herbal medicine. The objective of this study was to investigate the subacute toxic effects of trans-Cinnamaldehyde (Cinn; 2000 mg/kg/day) administered intrarectally for 15 consecutive days in male C57BL/6 mice, a route of administration not previously evaluated in this model. Mice were monitored for survival, preclinical signs, and behavior. Neurotoxic potential was assessed using a functional observational battery (FOB), ambulatory activity, and spontaneous locomotion. Physiological parameters included sperm motility, liver function, relative liver weight, and macroscopic and microscopic liver morphology. Food and water intake were recorded and considered in the analysis. Subacute intrarectal administration of Cinn did not produce neurotoxic, hepatotoxic, or reproductive adverse effects. The treatment was well tolerated and associated with reduced body weight gain without changes in food or water intake. In conclusion, these results support the safety of Cinn and suggest potential applications in metabolic research, particularly in the context of obesity.

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References

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