Assessments of membrane stabilizing and clot lysis capacity of Canna indica flower aqueous extract: In vitro study

Abstract

This study aims to investigate the membrane-stabilizing and clot-lysis activities of an aqueous extract derived from the flowers of Canna indica (CIFA) on human blood in vitro. In this experiment, erythrocyte lysis was induced by a hypotonic solution, and clot formation was created by incubating the blood sample at 37 °C for 45 minutes. We conducted experiments using varying amounts of acetyl salicylic acid 6.25–100 µL and a fixed amount of streptokinase 100 µL as the positive control groups. The group designated as the negative control treated the vehicle. CIFA was treated at doses ranging from 6.25 to 100 µL. CIFA demonstrated dose-dependent membrane stabilizing and clot lysis abilities. The CIFA exhibited a wide range of membrane stabilizing capabilities, with a percentage of 71.03 ± 0.02 at a concentration of 100 µL. The EC₅₀ of the test extract was calculated to be 47.43 ± 1.02 µL. In contrast, CIFA demonstrated clot lysis capabilities of 60.38 ± 2.37% at the same concentration, with the EC₅₀ of the test extract calculated at 91.42 ± 1.86 µL. CIFA demonstrated significant membrane stabilizing and clot lysis activity in human blood, suggesting that they could be promising candidates for the treatment of atherothrombosis.