Journal of Phytochemical Insights
type: channel
description: Journal of Phytochemical Insights is a peer-reviewed, open-access journal published by BioLuster Research Center Ltd. The journal focuses on advancing research in natural products derived from plants, including phytochemicals, plant extracts, and their applications in drug discovery and traditional medicine. It provides a platform for studies on herbal medicine, scientific validation of local medicinal practices, and computational approaches to phytochemistry and drug design. With a commitment to fostering interdisciplinary collaboration, the journal aims to bridge traditional knowledge and modern science, promoting innovation in phytochemical research and sustainable health solutions. ISSN (Online): 3080-0005 | ISSN-L: 3080-0005 Journal DOI: https://doi.org/10.71193/jpci Indexed by: Google Scholar, Crossref, Scilit, WorldCat, Semantic Scholar, ResearchRib, ScienceOpen, ROAD, Zenodo, OpenAIRE
publisher: BioLuster Research Center Ltd
language: en
publication name: Journal of Phytochemical Insights
issn: 3080-0005
items: AI integration in bioactive-oriented research: Discovery, development, and design for pharmaceutical and medical applications
items: Berberine Alleviates Anxiety, Depression, and Motor Impairment Associated with Alcohol Withdrawal in Mice: A Preclinical Study
items: Assessment of Phytochemical Screening, Antimicrobial, Antioxidant, and Thrombolytic Potential of Ethanolic Leaf Extract of Clerodendrum indicum
items: Assessments of membrane stabilizing and clot lysis capacity of Canna indica flower aqueous extract: In vitro study
items: Anticancer Activity of Scutellarein Against Several Cancer Types with Possible Mechanisms: A Mini Review
items: Anticancer Potential of Diazepam: Pharmacological Relevance and Clinical Evidence
items: Recent Advances in Plant‑Derived Anti‑Cancer Compounds: Promising Leads
items: Phytochemical Profiling and Evaluation of Antioxidant, Antimicrobial, and Thrombolytic Activities of Ethanolic Extract of Tarenna campaniflora Leaves
items: Sub-acute Cinnamaldehyde rectal administration induces non-toxic effects in neurological and physiological responses in C57BL/6 mice
items: Assessment of the Anticancer Activity of Aegle marmelos Leaf Extracts Against Ehrlich's Ascites Carcinoma (EAC) Cell Line in Swiss Albino Mice
items: Phytochemical Screening, FT-IR and GC-MS Analysis of Extracts from Aloe Aculeata Pole-Evans Leaves used for Managing Poultry Health in Drylands of Zimbabwe
items: Anti-diarrheal and thrombolytic activity of the leaf extract of Phyla nodiflora
items: Anticancer Potential of Oleandrin: Preclinical Mechanisms, Botanical Origins, and Pharmacokinetic Insights
items: Toxicological Profiling of Natural Bioactive Compounds Using PubChem Database and Relevant Literature
items: Apigenin: A Natural Flavonoid with Therapeutic Potential in Cancer Mechanisms, Physiochemical and Biopharmaceutical Properties, and Future Perspectives
items: Anticancer activity of Magnifera indica through the caspase activation: A short review
items: Assessment of Phytochemical Screening, Antioxidant, Anti-inflammatory, Hypoglycemic, Antidiarrheal, and Anxiolytic Effects of Curculigo orchioides Leaf Extracts
items: Research Perspectives on Plant Materials and Their Derivatives Align with the Journal of Phytochemical Insights

AI integration in bioactive-oriented research: Discovery, development, and design for pharmaceutical and medical applications
type: item
subject: Editorial
  topic: section
subject: Others
  topic: category
creator: Md Torequl Islam
rights: Copyright (c) 2026 Md Torequl Islam (Author) https://creativecommons.org/licenses/by/4.0
date: 2026-03-27
publication date: 2026-03-27
starting page: 1
ending page: 2
doi: 10.71193/jpci.20260017

Berberine Alleviates Anxiety, Depression, and Motor Impairment Associated with Alcohol Withdrawal in Mice: A Preclinical Study
type: item
description: Alcohol withdrawal is a serious condition that often leads to heightened anxiety, depressive symptoms, and problems with motor coordination, all of which can significantly affect quality of life. These effects are largely tied to chemical imbalances in the brain, particularly involving GABA and glutamate systems, as well as oxidative stress. Berberine, a natural plant alkaloid long recognized for its therapeutic potential, is known to have calming, mood-stabilizing, and neuroprotective properties. In this study, mice were exposed to repeated alcohol intake (10% v/v, 2 g/kg p.o) followed by withdrawal, which produced marked behavioral changes resembling anxiety, depression, and poor motor performance. When treated with berberine (10 or 30 mg/kg p.o.) , however, these animals showed a clear reversal of withdrawal-induced problems. At both lower and higher doses, berberine reduced anxiety-like behavior, improved exploratory activity, lessened depressive signs, and improved motor coordination, with the higher dose offering stronger benefits. Importantly, berberine by itself did not cause any harmful effects, suggesting that it is safe in this context. These findings indicate that berberine may offer meaningful protection against the emotional and motor disturbances caused by alcohol withdrawal. Its ability to ease anxiety and depression while supporting brain function makes it a promising candidate for managing alcohol withdrawal syndrome.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Neuroscience
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Berberine
  topic: keywords
subject: Alcohol withdrawal
  topic: keywords
subject: Anxiety
  topic: keywords
subject: Depression
  topic: keywords
subject: Motor coordination
  topic: keywords
creator: Jayesh Bagul
creator: Manojkumar Mahajan
creator: Aman Upaganlawar
creator: Chandrashekhar Upasani
rights: Copyright (c) 2025 Jayesh Bagul, Manojkumar Mahajan, Aman Upaganlawar, Chandrashekhar Upasani (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-12-11
publication date: 2025-12-11
starting page: 1
ending page: 7
doi: 10.71193/jpci.20250017

Assessment of Phytochemical Screening, Antimicrobial, Antioxidant, and Thrombolytic Potential of Ethanolic Leaf Extract of Clerodendrum indicum
type: item
description: Clerodendrum indicum is a traditional medicinal plant known for its therapeutic properties. Its leaves contain various bioactive compounds that may exhibit antimicrobial, antioxidant, and thrombolytic activities. This study aimed to identify major phytochemicals in C. indicum leaves and evaluate their antimicrobial, antioxidant, and thrombolytic effects. Ethanolic leaf extract was screened for phytochemicals using standard tests. Antimicrobial activity was assessed via the disc diffusion method against Gram-positive and Gram-negative bacteria. Antioxidant activity was evaluated using the DPPH assay. Thrombolytic activity was tested by measuring clot lysis in vitro and comparing it with streptokinase. Phytochemical screening confirmed the presence of alkaloids, tannins, saponins, reducing sugars, steroids, and gums. The extract showed moderate antibacterial activity, particularly against Salmonella enterica (27 mm at 500 µg/disc) and Streptobacillus moniliformis (24 mm at 500 µg/disc). Antioxidant analysis revealed a dose-dependent scavenging effect with an IC50 of 82 µg/mL. Thrombolytic activity showed 43.87% clot lysis at 20 mg/mL concentration, compared to 69.37% with streptokinase. C. indicum leaves possess promising bioactive properties, supporting their traditional use. The extract demonstrated moderate antimicrobial and thrombolytic effects and notable antioxidant potential. Further studies are needed to isolate specific compounds and understand their mechanisms.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Ptants
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Clerodendrum indicum
  topic: keywords
subject: Phytochemical screening
  topic: keywords
subject: Ethanolic extract
  topic: keywords
subject: Antimicrobial activity
  topic: keywords
subject: Antioxidant activity
  topic: keywords
subject: Thrombolytic activity
  topic: keywords
subject: DPPH assay
  topic: keywords
creator: Dipongkar Ray Sobuj
creator: Tamanna Rashid
creator: Asit Baron Sarker
creator: Monotosh Kumar Sarkar
creator: Rafiqul Islam
creator: Rumel Dey
creator: Chandana Rani Mridha
creator: Md Reaz Uddin
creator: Keerthi Gazula
creator: Iffat Ara Zabeen
creator: Salehin Sheikh
rights: Copyright (c) 2025 Dipongkar Ray Sobuj, Tamanna Rashid, Asit Baron Sarker, Monotosh Kumar Sarkar, Rafiqul Islam, Rumel Dey, Chandana Rani Mridha, Md Reaz Uddin, Keerthi Gazula, Iffat Ara Zabeen, Salehin Sheikh (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-11-24
publication date: 2025-11-24
starting page: 1
ending page: 10
doi: 10.71193/jpci.20250014

Assessments of membrane stabilizing and clot lysis capacity of Canna indica flower aqueous extract: In vitro study
type: item
description: This study aims to investigate the membrane-stabilizing and clot-lysis activities of an aqueous extract derived from the flowers of Canna indica (CIFA) on human blood in vitro. In this experiment, erythrocyte lysis was induced by a hypotonic solution, and clot formation was created by incubating the blood sample at 37 °C for 45 minutes. We conducted experiments using varying amounts of acetyl salicylic acid 6.25–100 µL and a fixed amount of streptokinase 100 µL as the positive control groups. The group designated as the negative control treated the vehicle. CIFA was treated at doses ranging from 6.25 to 100 µL. CIFA demonstrated dose-dependent membrane stabilizing and clot lysis abilities. The CIFA exhibited a wide range of membrane stabilizing capabilities, with a percentage of 71.03 ± 0.02 at a concentration of 100 µL. The EC50 of the test extract was calculated to be 47.43 ± 1.02 µL. In contrast, CIFA demonstrated clot lysis capabilities of 60.38 ± 2.37% at the same concentration, with the EC50 of the test extract calculated at 91.42 ± 1.86 µL. CIFA demonstrated significant membrane stabilizing and clot lysis activity in human blood, suggesting that they could be promising candidates for the treatment of atherothrombosis.
subject: Research Article
  topic: section
subject: Ptants
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Canna indica flower
  topic: keywords
subject: aqueous extract
  topic: keywords
subject: hypotonic solution
  topic: keywords
subject: atherothrombosis
  topic: keywords
creator: Raihan Chowdhury
creator: Md. Sakib Al Hasan
rights: Copyright (c) 2025 Raihan Chowdhury, Md. Sakib Al Hasan (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-01-01
publication date: 2025-01-01
starting page: 1
ending page: 7
doi: 10.71193/jpci.20250001

Anticancer Activity of Scutellarein Against Several Cancer Types with Possible Mechanisms: A Mini Review
type: item
description: Scutellarein (STN), a bioactive flavone found in various medicinal plants such as Scutellaria baicalensis and Scutellaria lateriflora. It has a variety of pharmacological actions, including anticancer properties. This study explores STN’s anticancer activity against various cancer cell lines. A literature review was conducted using databases such as PubMed, ScienceDirect, and Google Scholar, focusing on research evaluating STN’s anticancer efficacy. The results showed that STN has significant anticancer properties against various types of cancer, such as blood, colon, colorectal, gastric, liver, lung, ovarian, and renal cancers. STN reduces cell growth by cell cycle arrest, triggers apoptosis through caspase activation, and inhibits metastasis by suppressing mitochondrial membrane potential (MMP) and epithelial mesenchymal transition (EMT) markers. It modulates important pathways such as ERK, Wnt/β-catenin, and PI3K/Akt/NF-κB. Together, these systems prevent the growth and metastasis of cancer cells. The compound has exhibited prominent cytotoxicity in diverse cancer cell lines.
subject: Short Review
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Scutellarein
  topic: keywords
subject: Anti-cancer
  topic: keywords
subject: Proliferation
  topic: keywords
subject: Flavone
  topic: keywords
creator: Imam Hossen Rakib
creator: Rakib Hossan
creator: Md. Sakib Al Hasan
creator: Noshin Tasnim Yana
creator: Fowjia Ahmed Safa
creator: Umme Habiba Sumaya
rights: Copyright (c) 2025 Imam Hossen Rakib, Rakib Hossan, Md. Sakib Al Hasan, Noshin Tasnim Yana, Fowjia Ahmed Safa, Umme Habiba Sumaya (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-03-13
publication date: 2025-03-13
starting page: 1
ending page: 6
doi: 10.71193/jpci.20250005

Anticancer Potential of Diazepam: Pharmacological Relevance and Clinical Evidence
type: item
description: Cancer treatments such as chemotherapy, radiation, and targeted therapies often face challenges like severe side effects, drug resistance, and high costs. Repurposing existing medications is a promising strategy to improve treatment outcomes. Diazepam (DZP), a benzodiazepine commonly used for anxiety, has recently shown potential anticancer effects. This study explores the anticancer activity of DZP, focusing on its mechanisms of action, pharmacological relevance, immunotherapy, pharmacokinetics profile, toxicological profile, and clinical evidence through literature review from articles and journals in PubMed, Scopus, Google Scholar, and the Web of Science. DZP exerts antitumor effects by inducing apoptosis, inhibiting cell proliferation, and modulating oxidative stress and cell cycle dynamics against various cancers: blood cancer, brain cancer, breast cancer, colorectal cancer, glioblastoma, lung cancer, and skin cancer. DZP also demonstrates synergistic potential with conventional chemotherapeutic agents, enhancing their effectiveness while reducing patient anxiety and stress. Its pharmacokinetic profile, characterized by rapid absorption, extensive tissue distribution, and a prolonged half-life, supports its utility as a sustained anticancer agent. A significant gap in our investigation is the lack of clinical data on DZP; additional clinical evidence is required to validate its anticancer potential and establish it as an anticancer drug in the near future. Its sedative and CNS depressant effects necessitate cautious use, particularly in patients with hepatic or renal impairment. This review highlights the potential of DZP as a cost-effective, repurposed therapeutic option for cancer treatment. Additionally, it demonstrates how DZP may be a viable anticancer medication with a potential mechanism, maybe providing a better safety profile and less serious side effects than traditional treatments like chemotherapy.
subject: Review
  topic: section
subject: Drug Discovery and Development
  topic: category
subject: Cancer
  topic: keywords
subject: Diazepam
  topic: keywords
subject: Literature review
  topic: keywords
subject: Pharmacological relevance
  topic: keywords
creator: Md. Mizan
creator: Noshin Tasnim Yana
creator: Ali Mohamod Wasaf Hasan
creator: Mohammed Burhan Uddin
creator: Md Abu Sayeed
creator: Akayed Hasan
creator: Shafayatun Nahar Tinu
creator: Imam Hossen Rakib
creator: Md. Sakib Al Hasan
rights: Copyright (c) 2025 Md. Mizan, Noshin Tasnim Yana, Ali Mohamod Wasaf Hasan, Mohammed Burhan Uddin, Md Abu Sayeed, Akayed Hasan, Shafayatun Nahar Tinu, Imam Hossen Rakib, Md. Sakib Al Hasan (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-02-08
publication date: 2025-02-08
starting page: 1
ending page: 9
doi: 10.71193/jpci.20250003

Recent Advances in Plant‑Derived Anti‑Cancer Compounds: Promising Leads
type: item
subject: Editorial
  topic: section
subject: Others
  topic: category
creator: Md Shimul Bhuia
rights: Copyright (c) 2025 Md Shimul Bhuia (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-11-22
publication date: 2025-11-22
starting page: 1
ending page: 2
doi: 10.71193/jpci.20250016

Phytochemical Profiling and Evaluation of Antioxidant, Antimicrobial, and Thrombolytic Activities of Ethanolic Extract of Tarenna campaniflora Leaves
type: item
description: Discovery of the genus Tarenna campaniflora and its traditional uses was obtained by living in forest or semi-forest areas and by close observations of the indigenous populations. This study evaluated the phytochemical constituents, antioxidant potential, antimicrobial efficacy, and thrombolytic activity of ethanolic leaf extract of Tarenna campaniflora. Qualitative phytochemical screening revealed the presence of various bioactive metabolites. DPPH assay demonstrated notable antioxidant activity (IC50 = 0.877 µg/mL), comparable to ascorbic acid (IC50 = 0.839 µg/mL). Disc diffusion assay showed antimicrobial activity, with the highest inhibition against Escherichia coli (18 mm). The extract also exhibited clot lysis (22.42%), compared to streptokinase (31.17%). The findings support the therapeutic potential of T. campaniflora, warranting further pharmacological investigations.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Tarenna campaniflora
  topic: keywords
subject: phytochemical screening
  topic: keywords
subject: anti-microbial activity
  topic: keywords
subject: thrombolytic activity
  topic: keywords
creator: Md. Mizan
creator: Md. Shawon Miah
creator: Umme Habiba Sumaya
creator: Noshin Tasnim Yana
creator: Mst Muslima Khatun
creator: Proma Mandal
creator: Ali Mohamod Wasaf Hasan
creator: Mohammed Burhan Uddin
creator: Md Abu Sayeed
creator: Khadija Akter
creator: Mohammad Aslam
creator: Emon Mia
creator: Md. Sakib Al Hasan
rights: Copyright (c) 2025 Md. Mizan, Md. Shawon Miah, Umme Habiba Sumaya, Noshin Tasnim Yana, Mst Muslima Khatun, Proma Mandal, Ali Mohamod Wasaf Hasan, Mohammed Burhan Uddin, Md Abu Sayeed, Khadija Akter, Mohammad Aslam, Emon Mia, Md. Sakib Al Hasan (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-08-27
publication date: 2025-08-27
starting page: 1
ending page: 8
doi: 10.71193/jpci.20250011

Sub-acute Cinnamaldehyde rectal administration induces non-toxic effects in neurological and physiological responses in C57BL/6 mice
type: item
description: Trans-Cinnamaldehyde, the main component of cinnamon essential oil, is widely used in food and traditional herbal medicine. The objective of this study was to investigate the subacute toxic effects of trans-Cinnamaldehyde (Cinn; 2000 mg/kg/day) administered intrarectally for 15 consecutive days in male C57BL/6 mice, a route of administration not previously evaluated in this model. Mice were monitored for survival, preclinical signs, and behavior. Neurotoxic potential was assessed using a functional observational battery (FOB), ambulatory activity, and spontaneous locomotion. Physiological parameters included sperm motility, liver function, relative liver weight, and macroscopic and microscopic liver morphology. Food and water intake were recorded and considered in the analysis. Subacute intrarectal administration of Cinn did not produce neurotoxic, hepatotoxic, or reproductive adverse effects. The treatment was well tolerated and associated with reduced body weight gain without changes in food or water intake. In conclusion, these results support the safety of Cinn and suggest potential applications in metabolic research, particularly in the context of obesity.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Trans-Cinnamaldehyde
  topic: keywords
subject: intra-rectal
  topic: keywords
subject: functional observational battery
  topic: keywords
subject: toxicity
  topic: keywords
subject: mice
  topic: keywords
creator: Emanuel Bottino
creator: Yesica Paola Zaio
creator: Natalia Agustina Saavedra Larralde
creator: Victoria Belén Occhieppo
creator: Maria Paula Zunino
creator: Andrés Alberto Ponce
rights: Copyright (c) 2025 Emanuel Bottino, Yesica Paola Zaio, Natalia A. Saavedra Larralde , Victoria B. Occhieppo , M. Paula Zunino, Andres A. Ponce (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-10-16
publication date: 2025-10-16
starting page: 1
ending page: 9
doi: 10.71193/jpci.20250015

Assessment of the Anticancer Activity of Aegle marmelos Leaf Extracts Against Ehrlich's Ascites Carcinoma (EAC) Cell Line in Swiss Albino Mice
type: item
description: Cancer remains a significant health threat globally, and as a result, the demand for safer and more effective alternatives keeps growing. In this current study, we have assayed the anticancer activity of Aegle marmelos extract on Ehrlich Ascites Carcinoma (EAC) cells on Swiss Albino mice. Three extracts were prepared: a methanolic (APME), chloroform (APCE), and n-hexane (APHE) extract. These extracts were administered intraperitoneally in doses of 50 mg/kg and 100 mg/kg per dose on 6 consecutive days to mice. The anticancer activity was assessed from viable tumor cell count, with percentage (%) inhibition of cell growth, and changes in body weight of the mice that had been treated with extracts. Of all the extracts, APHE exhibited the most tumor growth inhibition (62.2% at 100 mg/kg), followed by APME (59.25%) and APCE (54.86%), compared to the standard chemotherapy drug doxorubicin (81.6% inhibition at 0.8 mg/kg/day). Mice treated with the extracts experienced less weight loss than the control group, with an approximate effect of 0.50 ± 0.06 g/day. A Brine Shrimp Lethality Bioassay demonstrated a cytotoxic dose-dependent impact, with LC50 values of 18.38, 36.79, and 46.45 micrograms per milliliter for n-hexane, methanolic, and chloroform extracts, respectively. The tumor suppression and cytotoxicity effects of Aegle marmelos extracts are probably attributable to the bioactive compounds such as flavonoids, alkaloids, and essential oils that have known apoptotic and antiproliferative activities. The extracts were not as effective as doxorubicin; however, the extracts’ natural composition and ability to reduce weight loss due to cancer indicate potential to be used in combination with or as an alternative to cancer-treating medications. Further research includes that mechanistic studies and clinical trials are needed to confirm their therapeutic potential.
subject: Research Article
  topic: section
subject: Ptants
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Aegle marmelos
  topic: keywords
subject: Plant Leaf Extracts Ehrlich’s Ascites Carcinoma (EAC)
  topic: keywords
subject: Anticancer activity
  topic: keywords
subject: Swiss Albino mice
  topic: keywords
creator: Md. Arafat Hossain
creator: Md. Takdir Hossain
rights: Copyright (c) 2025 Md. Arafat Hossain, Md. Takdir Hossain (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-09-05
publication date: 2025-09-05
starting page: 1
ending page: 10
doi: 10.71193/jpci.20250013

Phytochemical Screening, FT-IR and GC-MS Analysis of Extracts from Aloe Aculeata Pole-Evans Leaves used for Managing Poultry Health in Drylands of Zimbabwe
type: item
description: Due to limited veterinary facilities, farmers in remote areas of Zimbabwe rely heavily on herbal ethnoveterinary medicines as a feasible alternative to conventional veterinary operations. Medicinal plants may be the best prospects for developing drugs to treat animal health problems. The medicinal properties of medicinal plants are attributable to the existence of diverse bioactive compounds with varying compositions that arise as secondary metabolites. According to research conducted in the drylands of Zimbabwe's Masvingo province, Aloe aculeata was the most commonly utilized Aloe species for managing poultry health. However, the scientific basis for the curative properties of Aloe aculeata had not yet been established. As a result, this study was done to address the aforementioned knowledge gap with the objective of performing a preliminary investigation of potential phytochemicals and to identify and characterize potential phytochemicals using FT-IR analysis and the GC-MS technique. Tannins, phenolic compounds, flavonoids, and phlobatannins tests gave positive results across extracts from shade-dried and fresh leaves from all three solvents (hexane, distilled water, ethanol). Flavonoids had the highest concentration in the leaf samples (5.6%), followed by alkaloids (3.2%) and saponins (3.15%). FT-IR analysis confirmed the presence of phenols, amines, aromatics, alkynes, alkenes, and quinones due to the functional groups that were detected. GC-MS analysis showed the presence of 32 compounds, and 17 have reported pharmacological activity. The high number of phytochemicals and compounds with pharmaceutical value justifies the use of Aloe aculeata in managing poultry diseases. The findings of the present study offer the ethnomedical use of this plant for the development of herbal drugs for the management of poultry health.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Aloe aculeata
  topic: keywords
subject: poultry
  topic: keywords
subject: drylands
  topic: keywords
subject: ethnoveterinary
  topic: keywords
subject: phytochemical
  topic: keywords
subject: drug development
  topic: keywords
creator: Vimbai Gobvu
creator: Xavier Poshiwa
creator: Zakio Makuvara
creator: Mudadi Albert Benhura
rights: Copyright (c) 2025 Vimbai Gobvu, Dr Xavier Poshiwa, Zakio Makuvara, Professor Mudadi A. Benhura (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-08-11
publication date: 2025-08-11
starting page: 1
ending page: 15
doi: 10.71193/jpci.20250012

Anti-diarrheal and thrombolytic activity of the leaf extract of Phyla nodiflora
type: item
description: Phyla nodiflora (PNF), a medicinal plant, may offer a safer, more accessible alternative to synthetic drugs with fewer side effects. This study aimed to investigate the anti-diarrheal and thrombolytic properties of PNF leaf extract using in vivo and in vitro models, respectively. The anti-diarrheal activity of PNF was evaluated in Swiss albino mice using a castor oil-induced diarrhea model. Mice were administered PNF orally at 300 and 600 mg/kg, with loperamide (LOP) (3 mg/kg) as the positive control and distilled water (DW) as the negative control (NC). For thrombolytic activity, an in vitro clot lysis assay was conducted using human blood samples. Our result demonstrated that PNF possesses significant anti-diarrheal effects in a dose-dependent manner, reducing defecation by 27.27% and 63.64% at 300 and 600 mg/kg, respectively (p < 0.05), compared to 72.73% inhibition by LOP. Additionally, PNF exhibited notable clot lysis activity (44.59%), surpassing the NC (8.06%) but lower than streptokinase (STK) (74.25%) (p < 0.005). However, PNF leaf extract shows promising anti-diarrheal and thrombolytic activities, suggesting its potential as a natural therapeutic agent. Further research is needed to clarify its mechanisms and confirm clinical effectiveness.
subject: Research Article
  topic: section
subject: Ptants
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Diarrhea
  topic: keywords
subject: Loperamide
  topic: keywords
subject: Phyla nodiflora
  topic: keywords
subject: Streptokinase
  topic: keywords
subject: Thrombotic disorders
  topic: keywords
creator: Nishat Bahadur
creator: Asit Baron Sarker
creator: Dipongkar Ray Sobuj
creator: Feroz Khan Nun
creator: Khadija Akter
creator: Md. Arif Hossain
creator: Proma Mandal
creator: Farhana Israt Jahan
creator: Tamanna Rashid
creator: Mohammad Aslam
creator: Md. Sakib Al Hasan
rights: Copyright (c) 2025 Nishat Bahadur, Asit Baron Sarker, Dipongkar Ray Sobuj, Feroz Khan Nun, Khadija Akter Khadija Akter, Md. Arif Hossain, Proma Mandal, Farhana Israt Jahan, Tamanna Rashid, Mohammad Aslam, Md. Sakib Al Hasan (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-07-21
publication date: 2025-07-21
starting page: 1
ending page: 6
doi: 10.71193/jpci.20250009

Anticancer Potential of Oleandrin: Preclinical Mechanisms, Botanical Origins, and Pharmacokinetic Insights
type: item
description: Oleandrin (OLD) is a lipid-soluble cardiac glycoside derived mainly from the leaves of Nerium oleander (Apocynaceae), traditionally used in herbal medicine. Among the various parts of the plant, the leaves contain the highest concentration of OLD. Red-flowered varieties contain higher levels of cardiac glycosides. Although Thevetia peruviana (yellow oleander) belongs to the same family, it does not produce OLD. Recent studies show that OLD has significant anticancer potential against various cancers such as breast, prostate, lung, colon, pancreatic, endometrial cancers, melanoma, and osteosarcoma. It works by inducing apoptosis (programmed cell death) via caspase activation, arresting the cell cycle, reducing oxidative stress, and lowering mitochondrial membrane potential. These actions are regulated by key signaling pathways including Wnt/β-catenin, phosphatidylinositol 3-kinase/protein kinase B/nuclear factor-kappa B (PI3K/Akt/NF-κB), and extracellular signal-regulated kinase (ERK). Pharmacokinetic studies reveal that OLD is rapidly absorbed through the oral mucosa and gastrointestinal tract, crosses the blood-brain barrier, and has a half-life of about 2.3 hours. It is mainly metabolized in the liver and intestine, and excreted mostly through feces (66%) and partly through urine (8%). Due to its strong anticancer mechanisms, OLD shows promise as a novel anticancer agent. However, further in vivo studies and clinical trials are necessary to confirm its therapeutic efficacy and safety in humans. Future research should also focus on optimizing its pharmacokinetic properties and minimizing toxicity to enable its transition from experimental models to clinical application.
subject: Review
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Anticancer
  topic: keywords
subject: apoptosis
  topic: keywords
subject: cell proliferation
  topic: keywords
subject: cytotoxicity
  topic: keywords
subject: oleandrin
  topic: keywords
creator: Maisa Rahman
creator: Md. Shadin
creator: Jakia Sultana
creator: Md Sabbir Hosain
creator: Most. Israt Jahan Oni
rights: Copyright (c) 2025 Maisa Rahman, Md. Shadin, Jakia Sultana, Md Sabbir Hosain, Most. Israt Jahan Oni (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-07-20
publication date: 2025-07-20
starting page: 1
ending page: 7
doi: 10.71193/jpci.20250010

Toxicological Profiling of Natural Bioactive Compounds Using PubChem Database and Relevant Literature
type: item
description: Natural bioactive compounds have gained increasing attention for their pharmacological properties and therapeutic potential. However, understanding their toxicological profiles is crucial for assessing their safety and potential applications. This study aimed to assess the toxicological profiles of selected natural compounds using LD50 values and literature-based evaluations. Toxicity data were collected from the PubChem database and scientific literature, covering various administration routes and model organisms. The toxicological assessment revealed varying LD50 values among bioactive compounds, with toxicity influenced by structure, organism, and administration route. Compounds like ellagic acid, swertiamarin, and verbascoside exhibited high safety margins, whereas angelicin, xanthotoxol, and senegenin showed lower LD50 values, indicating greater toxicity. Route of administration significantly influenced toxicity; for example, bakuchiol and osthol were more toxic via intraperitoneal or intravenous injection than oral administration. Literature findings further supported these trends, highlighting species and dose-dependent effects. Some compounds also showed model-specific toxicity despite therapeutic potential. Toxicity varied significantly depending on the route of administration; for example, bakuchiol and osthol exhibited higher toxicity when administered intraperitoneally or intravenously compared to oral administration, indicating the importance of the administration route in toxicity profiling.
subject: Review
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Toxicity analysis
  topic: keywords
subject: Lethal dose 50
  topic: keywords
subject: Bioactive compound
  topic: keywords
subject: Natural sources
  topic: keywords
creator: Md. Sakib Al Hasan
creator: Khadija Akter
creator: Mohammad Aslam
creator: Proma Mandal
creator: Imam Hossen Rakib
creator: Nasimul Haque Shipon
rights: Copyright (c) 2025 Md. Sakib Al Hasan, Khadija Akter, Mohammad Aslam, Proma Mandal, Imam Hossen Rakib, Nasimul Haque Shipon (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-07-13
publication date: 2025-07-13
starting page: 1
ending page: 8
doi: 10.71193/jpci.20250008

Apigenin: A Natural Flavonoid with Therapeutic Potential in Cancer Mechanisms, Physiochemical and Biopharmaceutical Properties, and Future Perspectives
type: item
description: Apigenin (APG), a flavonoid found in various fruits, vegetables, and herbs, has gained attention for its anticancer properties. This review summarizes the various botanical origins, physiochemical & biopharmaceutical properties of APG and its anticancer activity across different cancer types by investigating its effects on various cancer cell lines, underlying mechanisms, and effective doses. Studies indicate that APG exerts its anticancer effects by inhibiting cell proliferation, inducing apoptosis, and promoting autophagy. Additionally, APG has been shown to trigger necroptosis in cancer cells, contributing to its anticancer potential. The data highlights the ability of APG to suppress cancer cell growth, migration, and invasion, with varying effective doses depending on the cancer type. This review exhibits the potential of APG as a promising anticancer agent, revealing its therapeutic applications. However, further research, particularly clinical trials, is needed to optimize dosing and assess its full therapeutic potential in cancer treatment
subject: Short Review
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Apigenin
  topic: keywords
subject: Anti-Cancer Activity
  topic: keywords
subject: Flavonoids
  topic: keywords
subject: Mechanism of action
  topic: keywords
subject: Pharmacokinetic
  topic: keywords
creator: Jakia Sultana
creator: Most. Israt Jahan Oni
creator: Md. Shadin
rights: Copyright (c) 2025 Jakia Sultana, Most. Israt Jahan Oni , Md. Shadin (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-04-08
publication date: 2025-04-08
starting page: 1
ending page: 8
doi: 10.71193/jpci.20250007

Anticancer activity of Magnifera indica through the caspase activation: A short review
type: item
description: Cancer is one of the major global health concerns. Although several treatment processes are available for cancer, they have many side effects. Several natural plants, like Mangifera indica, have gained attention for cancer treatment due to their fewer side effects. This paper aims to review the anticancer effects of M. indica by analyzing its role in activating caspase. A literature review was carried out using databases like PubMed, Science Direct, and Google Scholar directly, with a focus on in vitro and in vivo investigations to demonstrate caspase activation in cancer treatment. Our findings revealed that M. indica extracts significantly enhance caspase-3, -8, and -9 activity, which induces apoptosis in breast, cervical, and colon cancer cells. This plant extract fights against cancer by stopping the spread of cancer cells and their growth. M. indica exhibited significant anti-cancer activity via caspase activation, proving its potential as a natural anti-cancer therapy.
subject: Short Review
  topic: section
subject: Natural Compound
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Cancer
  topic: keywords
subject: Caspase activation
  topic: keywords
subject: Magnifera indica
  topic: keywords
subject: Natural sources
  topic: keywords
creator: Sumaya Akter Bithi
creator: Afiya Ajmee
creator: Md Faisal Amin
creator: Moushumi Afroza Mou
creator: Rituparna Biswas Suma
creator: Rakib Hossan
creator: Md. Arif Hossain
creator: Abida Sultana
rights: Copyright (c) 2025 Sumaya Akter Bithi, Afiya Ajmee, Md Faisal Amin, Moushumi Afroza Mou, Rituparna Biswas Suma, Rakib Hossan, Md. Arif Hossain, Abida Sultana (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-03-06
publication date: 2025-03-06
starting page: 1
ending page: 6
doi: 10.71193/jpci.20250004

Assessment of Phytochemical Screening, Antioxidant, Anti-inflammatory, Hypoglycemic, Antidiarrheal, and Anxiolytic Effects of Curculigo orchioides Leaf Extracts
type: item
description: Curculigo orchioides Gaertn., commonly known as Talamuli in Bangladesh and widely distributed across Asia, is traditionally used in Ayurvedic and Chinese medicine as an aphrodisiac and for treating asthma and jaundice. Our study aimed to perform a comparative phytochemical analysis and evaluation of some biological effects using in vitro and in vivo approaches of its leaf’s ethanol (ECO), n-hexane (HCO), and chloroform (CCO) fractions. After extraction and fractionation, a phytochemical analysis was followed by different tests for anti-bacterial, antioxidant, anti-inflammatory, anti-diabetic, anti-diarrheal, and anxiolytic effects. Phytochemical screening revealed high concentrations of alkaloids, glycosides, tannins, and reducing sugars. Acute toxicity tests confirmed ECO’s safety up to 3000 mg/kg in Swiss mice. Antibacterial assays indicated ECO and CCO exhibited moderate inhibition against tested bacteria, while HCO showed no activity. HCO demonstrated superior antioxidant, α-amylase inhibitory, and anti-diarrheal effects, whereas CCO effectively suppressed protein denaturation, stabilized membranes, and exhibited significant hypoglycemic and anxiolytic effects. Taken together, C. orchioides might be a good source of phytotherapeutic agents.
subject: Research Article
  topic: section
subject: Natural Compound
  topic: category
subject: Ptants
  topic: category
subject: Drug Discovery and Development
  topic: category
subject: Curculigo orchioides
  topic: keywords
subject: Leaf extract
  topic: keywords
subject: Pharmacological investigations
  topic: keywords
subject: Phytochemical analysis
  topic: keywords
subject: Talamuli
  topic: keywords
creator: Tuz Mia Nur Akhi
creator: Tawhida Islam
creator: Irin Sultana
creator: Abdullah Al Faruq
creator: M Mohi Uddin Chowdhury
creator: Md. Abid Hasan
creator: Imam Hossen Rakib
creator: Ali Mohamod Wasaf hasan
rights: Copyright (c) 2025 Tuz Mia Nur Akhi, Tawhida Islam, Irin Sultana, Abdullah Al Faruq, M Mohi Uddin Chowdhury, Md. Abid Hasan, Imam Hossen Rakib, Ali Mohamod Wasaf hasan (Author) https://creativecommons.org/licenses/by/4.0
date: 2025-03-28
publication date: 2025-03-28
starting page: 1
ending page: 12
doi: 10.71193/jpci.20250006

Research Perspectives on Plant Materials and Their Derivatives Align with the Journal of Phytochemical Insights
type: item
subject: Editorial
  topic: section
subject: Others
  topic: category
creator: Muhammad Torequl Islam
rights: Copyright (c) 2025 Muhammad Torequl Islam (Author)
date: 2025-01-01
publication date: 2025-01-01
starting page: 1
ending page: 2
doi: 10.71193/jpci.20250002