Gas Chromatography Mass Spectroscopy (GC-MS) Fingerprinting of Methanol Extract and Ethanol-based Fractions of Corchorus olitorious (Jute) Leaves

Martin Msughter Ganyam; Sule Ola  Salawu; Afolabi A  Akindahunsi

doi:10.71193/jcid.20250008

Authors

Martin Msughter Ganyam Department of Biochemistry, Federal University of Technology, Akure, Nigeria Author https://orcid.org/0000-0002-6066-711X
Sule Ola Salawu Department of Biochemistry, Federal University of Technology, Akure, Nigeria Author https://orcid.org/0000-0002-6986-1234
Afolabi A Akindahunsi Department of Biochemistry, Federal University of Technology, Akure, Nigeria Author https://orcid.org/0000-0003-1969-1967

DOI:

https://doi.org/10.71193/jcid.20250008

Keywords:

Chromatography, Phytochemicals, Medicinal chemistry, Terpenes, Corchorus olitorious

Abstract

Gas Chromatography-Mass Spectrometry (GC-MS) is a powerful analytical technique used to identify and quantify bioactive compounds in complex mixtures like plant extracts. The study, therefore, aimed at fingerprinting bioactive compounds of crude and saponin-rich fractions of Corchorus olitorius leaves using qualitative analysis and Gas Chromatography Mass Spectroscopy (GC-MS). Qualitative screening revealed moderate levels of phenols, tannins, flavonoids, glycosides, and alkaloids in the crude and most fractions. The compounds with major peaks for GC-MS were: Fumaric acid, 2 methylpentyl 2,3-dichlorodichlorophenyl ester,4-Methyl-6-phenylpyrimidin-2-yl)(4,6,8-trimethylquinazolin-2-yl) amine, Fumaric acid, 4-heptyl tridecyl ester, and Dodecanoic acid, 1,2,3-propanetriyl ester in the crude extract. Ethanol 100 % fraction also revealed 4-Methoxy-3-nitrobenzyl alcohol, 4,4'-Bi-1,3-dioxolane, 2,2,2',2'-tetramethyl- and 2-(2 (2-Methoxyethoxy) ethoxy) ethyl pentanoate. While the Ethanol/methanol 80:20 fraction had Hexadecanoic acid methyl ester, Lauric anhydride, Citral, 2,6-Octadienal 3,7-dimethyl-, (Z)-. Ethanol/methanol 60:40 fraction further revealed Geranic acid, n Hexadecanoic acid, Hexadecanoic acid, methyl ester, and 1,4-Cyclohexadiene, 6 isopropenyl-2,4-dimethyl-1,3-bis(trimethylsilyl)-. The methanol crude extract and ethanol-based fractions of Corchorus olitorius leaves have revealed diverse classes of chemical compositions, which may be harnessed for therapeutic purposes.

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Author Biographies

Martin Msughter Ganyam, Department of Biochemistry, Federal University of Technology, Akure, Nigeria

Martin Msughter Ganyam is a lecturer with Joseph Sarwuan Tarka University, in the Department of Biochemistry. His area of research interest is Medicinal Biochemistry and currently a PHD student from the Federal University of Technology, Akure, Ondo State, Nigeria.
Sule Ola Salawu, Department of Biochemistry, Federal University of Technology, Akure, Nigeria

Sule Ola Salawu is currently a Professor in the Department of Biochemistry with specialization in Food Biochemistry from the Federal University of Technology, Akure, Ondo State, Nigeria
Afolabi A Akindahunsi, Department of Biochemistry, Federal University of Technology, Akure, Nigeria

Afolabi A Akindahunsi is currently a Professor in the Department of Biochemistry with specialization in Food Biochemistry from the Federal University of Technology, Akure, Ondo State, Nigeria.

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